Fig. 3: NapBu–BPEA distributes to multiple organelles in HeLa cells. | Nature Communications

Fig. 3: NapBu–BPEA distributes to multiple organelles in HeLa cells.

From: Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Fig. 3

a–l SIM images for HeLa cells co-stained by NapBu–BPEA (10 μM) with a–c LysoTracker Red (LTR, 0.1 μM), d–f MitoTracker Deep Red (MTDR, 0.5 μM), g–i ER-Tracker Red (ERTR, 1 μM), j–l nuclear dye Hoechst 33258 (Hoechst, 1 μg/mL). All insets give the enlarged images for the dashed pane. m Confocal (i and iii) and SIM (ii and iv) images for the NapBu-BPEA-stained HeLa cells, and n the fluorescence signal detected on the lines across a specific mitochondrion in (iii) and (iv). Scale bar: 5 μm, enlarged images scale bar: 0.5 μm.

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