Fig. 5: NapBu-BPEA reveals labile Zn2+ dynamics in autophagic HeLa cells. | Nature Communications

Fig. 5: NapBu-BPEA reveals labile Zn2+ dynamics in autophagic HeLa cells.

From: Simultaneous Zn2+ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells

Fig. 5

SIM images of HeLa cells stained by NapBu–BPEA and DAPRed without (a–d) or with (e–h) CCCP (10 μM, 24 h, 37 °C) treatment. a, e cell images from the green channel for NapBu–BPEA (10 μM, 1 h, 37 °C) fluorescence; b, f cell images from the red channel for DAPRed (1 μM, 30 min, 37 °C) fluorescence; c, g merged images of red and green channel images; (d, h) cell images for cells in a, b underwent TPEN (100 μM, 30 min, 37 °C) treatment; i average fluorescence intensity recorded in the green channel images for cells with or without CCCP treatment. n = 6 biologically independent experiments per group, mean ± SD, the statistical differences between the experimental groups were analyzed by double-tailed Student’s t test, when p < 0.05, it was considered to have statistical significance. Scale bar, 5 μm. Source data are available as a Source Data file.

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