Fig. 4: THOC1 and THOC2 specifically bind precursor transcripts from long intronless and long-first-exon pachytene piRNA clusters. | Nature Communications

Fig. 4: THOC1 and THOC2 specifically bind precursor transcripts from long intronless and long-first-exon pachytene piRNA clusters.

From: Long first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes

Fig. 4

a RIP-seq was used to measure the abundance of RNA bound to THOC1 (n = 5) or THOC2 (n = 3) compared to IgG control (n = 4) in adult testis. Each dot represents the mean abundance normalized for transcript length (RPKM). b Heatmaps report the enrichment of THOC1 or THOC2 binding, measured by RIP-seq, relative to IgG control for pachytene piRNA clusters in adult testis. TSS, transcription start site. Each row corresponds to pachytene piRNA gene, in first-exon (spliced) or transcript (unspliced) length order. For each gene, the black bar denotes the first exon–intron boundary; the blue bar indicates the 3′ end of the transcript.

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