Fig. 4: CYK-1 and PFN-1 act downstream of the CYB-3 signal pathway in the EHEC-induced ACT-5 mislocalization.
From: Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli
a The percentage of ACT-5 mislocalization in mC::ACT-5 animals subjected to RNAi of various actin modifiers and fed with OP50 or EHEC for 4 days were examined. b The quantification results of ACT-5 mislocalization in wild-type (WT) and cyk-1 overexpressing (cyk-1 o/e) animals exposed to OP50 or EHEC for 3 and 4 days. 3 DPI (WT-OP50 group: n = 111, N = 3; cyk-1 o/e-OP50: n = 125, N = 3, P = 0.6089; WT-EHEC group: n = 116, N = 3; cyk-1 o/e-EHEC: n = 120, N = 3, P = 0.0289); 4 DPI (WT-OP50 group: n = 110, N = 3; cyk-1 o/e-OP50: n = 118, N = 3, P = 0.4531; WT-EHEC group: n = 108, N = 3; cyk-1 o/e-EHEC: n = 107, N = 3, P = 0.019). c The percentage of ACT-5 mislocalization of wild-type (WT);mC::ACT and pfn-1(ok808);mC::ACT-5 animals fed with OP50 or EHEC at 20 °C for 4 days were examined. WT-OP50 group: n = 109, N = 3; pfn-1(ok808)-OP50: n = 96, N = 3, P = 0.3739; WT-EHEC group: n = 91, N = 3; pfn-1(ok808)-EHEC: n = 100, N = 3, P = 0.011. d The percentage of ACT-5 mislocalization was examined in the mC::ACT-5 animals with wild-type (WT) and cyb-3 overexpressing (cyb-3 o/e) background treated with cyk-1 or pfn-1 RNAi and fed with OP50 at 20 °C for 3 or 4 days post infection. 3 DPI (WT-L4440: n = 107, N = 3; WT-cyk-1: n = 120, N = 3, P = 0.4573; WT-pfn-1: n = 106, N = 3, P > 0.9999; cyb-3 o/e-L4440: n = 122, N = 3; cyb-3 o/e-cyk-1: n = 94, N = 3, P = 0.7953; cyb-3 o/e-pfn-1: n = 104, N = 3, P = 0.3739); 4 DPI (WT-L4440: n = 106, N = 3; WT-cyk-1: n = 106, N = 3, P > 0.9999; WT-pfn-1: n = 106, N = 3, P = 0.4353; cyb-3 o/e-L4440: n = 121, N = 3; cyb-3 o/e-cyk-1: n = 103, N = 3, P = 0.4929; cyb-3 o/e-pfn-1: n = 119, N = 3, P = 0.3486). e The percentage of ACT-5 mislocalization was examined in the mC::ACT-5 animals with wild-type (WT) and cyb-3 overexpressing (cyb-3 o/e) background treated with cyk-1 or pfn-1 RNAi and fed with EHEC at 20 °C for 3 or 4 days. 3 DPI (WT-L4440: n = 92, N = 3; WT-cyk-1: n = 97, N = 3, P = 0.325; WT-pfn-1: n = 123, N = 3, P = 0.0704; cyb-3 o/e-L4440: n = 107, N = 3; cyb-3 o/e-cyk-1: n = 84, N = 3, P = 0.0172; cyb-3 o/e-pfn-1: n = 113, N = 3, P = 0.0117); 4 DPI (WT-L4440: n = 84, N = 3; WT-cyk-1: n = 101, N = 3, P = 0.0033; WT-pfn-1: n = 104, N = 3, P = 0.0038; cyb-3 o/e-L4440: n = 84, N = 3; cyb-3 o/e-cyk-1: n = 82, N = 3, P = 0.0058; cyb-3 o/e-pfn-1: n = 102, N = 3, P = 0.0026). f The percentage of ACT-5 mislocalization were examined in the mC::ACT-5 worms with WT and cyk-1-overexpressing (cyk-1 o/e) background and treated with cyb-3 or pfn-1 RNAi feeding on OP50 at 20 °C, for 3 or 4 days. 3 DPI (WT-L4440: n = 106, N = 3; WT-cyb-3: n = 106, N = 3, P > 0.9999; WT-pfn-1: n = 84, N = 3, P > 0.9999; cyk-1 o/e-L4440: n = 78, N = 3; cyk-1 o/e-cyb-3: n = 78, N = 3, P = 0.8512; cyk-1 o/e-pfn-1: n = 84, N = 3, P = 0.3739); 4 DPI (WT-L4440: n = 101, N = 3; WT-cyb-3: n = 105, N = 3, P = 0.3453; WT-pfn-1: n = 80, N = 3, P = 0.5807; cyk-1 o/e-L4440: n = 85, N = 3; cyk-1 o/e-cyb-3: n = 74, N = 3, P = 0.1234; cyk-1 o/e-pfn-1: n = 84, N = 3, P = 0.9415). g The percentage of ACT-5 mislocalization was examined in the mC::ACT-5 worms with WT and cyk-1 overexpressing (cyk-1 o/e) background and treated with cyb-3 or pfn-1 RNAi fed on EHEC at 20 °C for 3 or 4 days. 3 DPI (WT-L4440: n = 103, N = 3; WT-cyb-3: n = 111, N = 3, P = 0.0002; WT-pfn-1: n = 105, N = 3, P = 0.0061; cyk-1 o/e-L4440: n = 76, N = 3; cyk-1 o/e-cyb-3: n = 75, N = 3, P = 0.0016; cyk-1 o/e-pfn-1: n = 95, N = 3, P = 0.0025); 4 DPI (WT-L4440: n = 101, N = 3; WT-cyb-3: n = 108, N = 3, P = 0.0021; WT-pfn-1: n = 103, N = 3, P = 0.0086; cyk-1 o/e-L4440: n = 71, N = 3; cyk-1 o/e- cyb-3: n = 73, N = 3, P = 0.0001; cyk-1 o/e-pfn-1: n = 88, N = 3, P = 0.0015). h Survival curves of N2 animals with RNAi of cdk-1, cyb-3, cyk-1, or pfn-1 genes and fed with EHEC. i Survival curves of wild-type N2, cyb-3-overexpressing (cyb-3 o/e), cyk-1-overexpressing (cyk-1 o/e), and cdk-1(ne2257) animals infected with EHEC at 20 °C were examined. For the survival analyses: *P < 0.05, **P < 0.01, and ***P < 0.001 by the Mantel–Cox log-rank test. The other quantitative data are presented as mean ± SEM, and each dot represented an independent result in the bar chart. Except for the survival analyses, all data statistics based on: *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired t test (two-tailed). Source data are provided as a Source Data file.
