Fig. 2: ZBP1 promotes CASP8-mediated cell death and IL-1β release.
From: ZBP1 promotes LPS-induced cell death and IL-1β release via RHIM-mediated interactions with RIPK1
a Schematic of protein domains in TRIF, RIPK1, and ZBP1. b, c Cell death over time in indicated BMDMs stimulated with LPS/5z7 as measured by (b) propidium iodide (PI) incorporation or (c) LDH release. d Cell death over time as measured by PI incorporation in B6 and Zbp1−/− BMDMs treated as indicated. e Full-length and cleaved products of indicated caspases and GSDMD from whole-cell lysates (WCL) or precipitated from the supernatant (Sup.) of B6 or Zbp1−/− BMDMs stimulated with LPS/5z7 for 1–6 h. f Levels of total, pRIPK1 (S166), and pRIPK1 (S321) in B6 or Zbp1−/− BMDMs stimulated with LPS or LPS/5z7 for 1–3 h. g Full-length and cleaved products of CASP1 and IL-1β from whole-cell lysates of B6 and Zbp1−/− BMDMs stimulated as indicated for 1–3 h. h IL-1β release after 6 h and (i) percentage of ASC+ cells after 2 h in B6, Zbp1−/−, and Ripk3−/−Casp8−/− BMDMs stimulated as indicated. Data from cell death assays and western blots are representative of 3 or more biologically independent experiments, cell death data are presented as the mean ± SD of triplicate wells, n = 10,000 cells examined in three individual wells. IL-1β release, ASC percentage, and qPCR data are presented as the mean ± SD for triplicate wells from n = 4 biologically independent experiments. Two-way analysis of variance (ANOVA) was used for comparison between groups. Source data for all experiments are provided as a Source data file. See also Supplementary Figs. 2 and 3.
