Fig. 3: ZBP1 promotes cell death via constitutive binding to RIPK1.
From: ZBP1 promotes LPS-induced cell death and IL-1β release via RHIM-mediated interactions with RIPK1
a ZBP1 immunoprecipitation in B6 BMDMs stimulated as indicated for 2 h and probed for RIPK1 and CASP8. b, c Schematic of full-length and mutant ZBP1 constructs generated, and confirmation of reconstitution experiments in Zbp1−/− BMDMs. d, e Cell death over time as measured by propidium iodide incorporation in Zbp1−/− BMDMs reconstituted with indicated constructs and treated with LPS/5z7. f FLAG-specific immunoprecipitation in Zbp1−/− BMDMs reconstituted with indicated ZBP1 constructs, stimulated with LPS or LPS/5z7 for 2 h and probed for ZBP1, RIPK1, and CASP8. Data from cell death assays and western blots are representative of 3 or more biologically independent experiments, cell death data are presented as the mean ± SD of triplicate wells, n = 10,000 cells examined in three individual wells. Source data for all experiments are provided as a Source data file. See also Supplementary Fig. 4.
