Fig. 4: TRIF induces complex formation independently of TNFR1.
From: ZBP1 promotes LPS-induced cell death and IL-1β release via RHIM-mediated interactions with RIPK1
a, b Cell death over time in indicated BMDMs stimulated with LPS/5z7 as measured by a propidium iodide (PI) incorporation or b LDH release. c–e Cell death over time as measured by PI incorporation in indicated BMDMs treated with c LPS/5z7+/− TNF-neutralizing antibody, d 5z7+/− TNF-neutralizing antibody or e Dynasore endocytosis inhibitor. f–h FADD immunoprecipitation in f B6, g Trif−/−, and h Tnfr−/− BMDMs stimulated as indicated and probed for ZBP1, RIPK1, and CASP8. i IL-1β release after 6 h in indicated BMDMs treated with LPS, LPS pre-primed LPS/5z7, or LPS pre-primed LPS/5z7 in the presence of NLRP3 inflammasome inhibitor MCC950. Data from cell death assays and western blots are representative of 3 or more biologically independent experiments, cell death data are presented as the mean ± SD of triplicate wells, n = 10,000 cells examined in three individual wells. IL-1β release data are presented as the mean ± SD for triplicate wells from n = 4 biologically independent experiments. Two-way analysis of variance (ANOVA) was used for comparison between groups. Source data for all experiments are provided as a Source data file.
