Fig. 5: Knock-down of ZEB2, TMSB4, and PTMA in cardiomyocytes inhibits endothelial cell migration and proliferation.

a Experimental setup. b Representative images of sprouts formed by HUVECs upon treatment indicated in (a). c Quantification of sprouts number per spheroid. d Quantification of cumulative sprout length per spheroid. Data in b-d is from one experiment with 4-12 biological replicates. e Representative immunofluorescent images of HUVECs treated as indicated in (a), nuclei stained for DAPI and EdU. f Quantification of EdU positive nuclei. g-i mRNA expression levels of g Mki67, h Pcna i Cdk4 in HUVECs treated with conditioned medium from (a). Data are represented as mean ± SEM, each dot indicates a biological replicate, n is indicated in figures. Data were analyzed by ordinary one-way ANOVA with Dunnett’s multiple comparison test (c, d, f, g, h, i). Source data are provided as a Source Data file.