Fig. 8: AAV9-mediated ZEB2 cardiac delivery improves infarct healing and cardiac function 28 dpMI.
From: Cardiomyocytes stimulate angiogenesis after ischemic injury in a ZEB2-dependent manner
a Study design. b-c Expression levels of b exogenous Zeb2 (CT values) and c Zeb2 mRNA in AAV9-control and AAV9-Zeb2 treated mice post-surgery. d Immunofluorescence for ZEB2, TNNT2 and DAPI of histological sections of hearts from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI. e-f Quantification of e fractional shortening (FS) and f left ventricular internal diameter in systole (LVIDs) from AAV9-control and AAV9-Zeb2 treated mice post-surgery. g-h mRNA expression levels of g Tmsb4 and h Ptma in hearts from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI. i-k Immunofluorescence for i TMSB4, j PTMA and k PECAM1, TNNT2 and DAPI of histological sections of hearts from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI. l-m mRNA expression levels of l Vegf and m Pecam1 in hearts from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI. n Quantification of PECAM1 positive blood vessel areas in histological sections of hearts from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI. o Representative WB for BCL-XL in tissue from AAV9-control and AAV9-Zeb2 treated mice 28 dpMI and p its quantification. q WGA staining to measure cardiomyocyte surface area and r its quantification. Green dashed line indicates sham AAV9-control. White arrows show ZEB2 positive cardiomyocytes. Data are represented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, each dot indicates a biological replicate, n is indicated in figures. Comparison of two groups was performed with the unpaired, two-tailed Student’s t-test between AAV9-Zeb2 vs AAV9-control post-MI (b, c, e, f, g, h, l, m, n, p, r). Source data are provided as a Source Data file.
