Fig. 3: Highly selective proximity proteomic analysis of stable protein complex in cytosol. | Nature Communications

Fig. 3: Highly selective proximity proteomic analysis of stable protein complex in cytosol.

From: Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Fig. 3

a Scheme of BN2-based proximity proteomic analysis in living cells. The well-characterized stable ILK-PINCH-PARVIN-RSU1 protein complex are shown (insert). b, c Volcano plot comparison between BP5- and BN2-based proximity proteomic analysis with stably expressed APEX2-FLAG-ILK in HT1080 cells as the bait protein (n = 3 independent biological experiments). d, e Reciprocal BN2-based proximity proteomic analysis of RSU1 (d) or PINCH (e) interactome. Stably expressed GFP was used as control in b–e. Significantly changed proteins in b–e were highlighted in red (FDR < 0.05 and S0 = 0.5). f LFQ intensity comparison of the ILK-PINCH-PARVIN-RSU1 complex proteins identified by the BP5- and BN2-based proximity proteomic analysis with ILK as bait protein. Each dot represents the indicated data of one independent experiment. Data are presented as mean values ± s.d. Error bars represent s.d. (n = 3 independent biological experiments). Source data are provided as a Source Data file.

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