Fig. 6: Spatiotemporal STS1 interactome at late time points of EGF stimulation. | Nature Communications

Fig. 6: Spatiotemporal STS1 interactome at late time points of EGF stimulation.

From: Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Fig. 6

a, b Overview and subcellular localization of STS1-interacting proteins that pass the data analysis cutoff only in APEX2-BP5 proximity proteomics. c The temporal curves of four proteins which are pivotal for membrane protein trafficking and endosome targeting. Data are presented as mean values ± s.d. (error bars; n = 3 independent biological experiments). d Colocalization of transiently co-transfected GFP-tagged RABEP1 with mCherry-tagged STS1 in HeLa cells upon EGF stimulation. HeLa cells were co-transfected with GFP-tagged RABEP1 and mCherry-tagged STS1 (n = 3 independent biological experiments). The scale bars are 10 µm and 50 µm (for zooming in). Results were quantitatively confirmed by line profile analysis shown in Supplementary Fig. 13c. Source data are provided as a Source Data file.

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