Fig. 2: YTHDF2 regulates GBM cell proliferation, invasion, and tumorigenesis. | Nature Communications

Fig. 2: YTHDF2 regulates GBM cell proliferation, invasion, and tumorigenesis.

From: EGFR/SRC/ERK-stabilized YTHDF2 promotes cholesterol dysregulation and invasive growth of glioblastoma

Fig. 2

a Cell viability of shCtrl and shYTHDF2 GSC11 cells was measured. b Proliferation of shCtrl and shYTHDF2 GSC11 cells was assessed by 5-bromo-2’-deoxyuridine (BrdU) incorporation for 3 h. The percentage of BrdU positive cells was quantified. c In vitro invasion assay for shCtrl and shYTHDF2 GSC11 cells. Representative invasion images of the cells (left panel) and quantitation of relative cell invasion (right panel). Scale bar = 50 μm. d Nude mice intracranial tumor assay using shCtrl, shYTHDF2, and shYTHDF2 plus shRNA-resistant-YTHDF2 GSC11 cells. Brain sections stained with hematoxylin and eosin (H&E) show representative tumor xenografts. Tumor volumes were calculated. e, f Quantification of Ki-67 (e) or cleaved Caspase-3 (f) positive cells in brain tumor sections of shCtrl, shYTHDF2, and shYTHDF2 plus shRNA-resistant-YTHDF2 GSC11 cells. g In vivo invasion assay for shCtrl, shYTHDF2, and shYTHDF2 plus shRNA-resistant-YTHDF2 GSC11 cells. Representative H&E staining showing edges of the mice brain tumors (left panel), and the relative invasive fingers per tumor were counted (right panel). Scale bar = 200 μm. h Overall survival of mice injected with the indicated GSC11 cells. i Nude mice intracranial tumor assay using LN229 cells with vector only or with YTHDF2 overexpression. Brain sections stained with H&E show representative tumor xenografts. Tumor volumes were calculated. j In vivo invasion assay for LN229 cells with vector only or with YTHDF2 overexpression. Representative H&E staining showing edges of the mice brain tumors (left panel), and the relative invasive fingers per tumor were summarized (right panel). Scale bar = 200 μm. k Overall survival of mice injected with the indicated LN229 cells. For a–c, data are mean ± S.E.M., n = 3 biologically independent experiments (one-way ANOVA Tukey’s post hoc test). For d–k, n = 10 mice per group examined over two independent experiments. For d–g, data are mean ± S.D. (one-way ANOVA Tukey’s post hoc test). For h and k, two-sided log-rank test is conducted. For i and j, data are mean ± S.D. (unpaired two-sided t test). Source data are provided as a Source data file.

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