Fig. 10: Enzyme activity and binding assays.
From: Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange
a Structures of substrate β-d-glucose and the studied inhibitors DTNB and (4)2 of glucose oxidase. b Lineweaver–Burk plots57 of DTNB and (4)2. Conditions: [glucose] from 0.2 to 4 mM, 37 °C at 50 mM sodium acetate buffer pH 5.15, [DTNB] 0.07 and 0.14 µM, [(4)2] 0.7 and 1.4 µM. Mean ± SD from four independent experiments. c Summary of the binding affinity and enzymatic activity parameters (Supplementary Methods). Source data are provided as Source Data file.
