Fig. 6: Kinetic rates to Secox.
From: Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange
a Homodimerization of building block 3 to (3)2. b Time course for the (3)2 formation using a concentration of 190 μM of (3), in 20 mM Tris buffer (pH 7.8), T = 6 °C, 0% (mol) Secox and 2.5% (v/v) DMSO (black dots), 5% (mol) TrxR and 2.5% (v/v) DMSO (light blue dots), 0% mol Secox and 20% (v/v) DMSO (purple dots), 1% (mol) Secox and 2.5% (v/v) DMSO (red dots), at 2.5% (mol) Secox and 2.5% (v/v) DMSO (green dots), 5% (mol) Secox and 2.5% (v/v) DMSO (blue dots) and 10% (mol) Secox and 2.5% (v/v) DMSO (orange dots). c Kinetic parameters. Reaction profiles were fitted to second-order reaction kinetic equations. Mean ± SD from three independent experiments. (Supplementary Fig. 38, Supplementary Methods, and Supplementary Discussion). Source data are provided as Source Data file.
