Fig. 7: Selenocystine applied to a slow DCL and a protein refolding.
From: Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange
a Slow thiol/disulfide exchange. Building block 5 (52,3 μM) and building blocks (6–8) at 104.6 μM concentration each. Tris buffer 20 mM pH 7.8 at 20% (v/v) DMSO and 6 °C. (i) 0% mol Secox as control after 192 h, (ii) 5% mol Secox (18.3 μM) after 60 h. Relative peak areas (RPAs) of the DCLs, see Supplementary Fig. 36 and Supplementary Table 2. pKa values estimated from Schrödinger Release 2020-254. See Supplementary Fig. 2 for pKa calculations. b Kinetics for scrambled RNase A folding at pH 7.8 and room temperature. Yellow circles symbolize disulfide bonds. General conditions: scrambled RNase A (5 μM), tris buffer 100 mM, pH 7.8, 2 mM EDTA. 0.2 mM GSSG/1 mM GSH (black dots), 1 mM Secox/5 mM GSH (red dots). Mean ± SD from two independent experiments. Source data are provided as Source Data file.
