Fig. 8: Protein engineering of VlmD for the enhanced production of ethylamine.
From: Microbial production of multiple short-chain primary amines via retrobiosynthesis
a Binding model of PLP-l-alanine in the homology model of VlmD. The homology model was generated using glutamate decarboxylase from Homo sapiens as a template. Potential six target residues for enzyme engineering are shown in cyan. b The concentrations of ethylamine produced by flask cultures of the EA01 strain and its derivative strains having one of the 12 different VlmD variants (Supplementary TableĀ 2). Experiments were conducted in triplicates. Data are presented as mean valuesāĀ±ās.d. Source data are provided as a Source Data file.
