Fig. 4: Whole body respirometry in male and female WT and adi-KO mice fed a chow or high-fat diet.
From: Deletion of Trim28 in committed adipocytes promotes obesity but preserves glucose tolerance
Trim28 wild type (WT) and adipose-specific Trim28 KO (KO) male and female mice were placed in metabolic cages (CLAMS) at 11 weeks of age (5 weeks post diet) for ~2 days. Respiratory exchange ratio (RER) over 24 h for chow-fed (a) male and (c) female mice, and average RER for light and dark periods for (b) male and (d) female mice. Energy expenditure assessed by ANCOVA (analysis of covariance) over 24 h for (e) chow-fed male and (f) female mice, and average activity, calculated as the sum of the average X/Y/Z beam breaks, for (g) male and (h) female chow-fed mice during light and dark periods. Respiratory exchange ratio (RER) over 24 h for (i) HFD-fed male and (k) female mice, and average RER for light and dark periods for (j) HFD-fed male and (l) female mice. Energy expenditure assessed by ANCOVA over 24 h for (m) HFD-fed male and (n) female mice, and average activity, calculated as the sum of the average X/Y/Z beam breaks, for (o) male and (p) female mice during light and dark periods. All data are represented as mean ± SEM, *p < 0.05 versus WT; p < 0.05 considered significant for ANCOVA analysis of energy expenditure. Chow-fed mice: female mice n = 11 WT and n = 11 KO, and male mice n = 12 WT and n = 11 KO. HFD-fed mice: female mice n = 12 WT and n = 12 KO, and male mice n = 11 WT and n = 9 KO. RER respiratory exchange ratio, (g) grams.
