Fig. 3: isoCirc characterization of circRNAs in 12 human tissues.
From: isoCirc catalogs full-length circular RNA isoforms in human transcriptomes
a Barplot showing number of full-length circRNA isoforms (y-axis) identified in each of 12 human tissues (x-axis), for read count = 1 (red), read count = 2 (blue), or read count ≥3 (green). b Correlation of number of circRNA BSJs identified from eight human tissues in published short-read datasets17 and isoCirc long-read datasets. For each tissue, number of unique circRNA BSJs identified in either short-read (x-axis) or long-read (y-axis) data was normalized by the total number of short reads or long reads in that tissue. Data were plotted as log10 transformed for ease of comparison and visualization. c Cumulative distribution plot of exon number per isoform for full-length circRNA isoforms with read count ≥2 in at least one of 12 human tissues. Isoforms were classified by their BSJ-FSJ categories, as follows: both BSJ and FSJs were FSM or NIC (red: FSM/NIC-FSM/NIC); BSJ was FSM or NIC, FSJs were NNC (blue: FSM/NIC-NNC); BSJ was NNC, FSJs were FSM or NIC (green: NNC-FSM/NIC); both BSJ and FSJs were NNC (purple: NNC–NNC); and all isoforms were combined (black: All–All). d Cumulative distribution plot of transcript length (nt) for full-length circRNA isoforms with read count ≥2 in at least one of 12 human tissues. Isoforms were classified by their BSJ-FSJ categories, as described in (c). e Heatmap showing numbers of genes with differential proportions of circRNA isoforms between each pair of 12 human tissues. f Heatmap showing numbers of circRNA isoforms with differential isoform proportions between each pair of 12 human tissues. g Stacked barplot showing isoform proportions of KDM1A circRNA isoforms across 12 human tissues. CircRNA isoforms were included in plot if read count was ≥2 in at least one tissue, and both BSJ and FSJs were FSM or NIC (FSM/NIC-FSM/NIC). Total read count for all circRNA isoforms in a tissue is given in parentheses on x-axis. h Stacked barplot showing isoform proportions of METTL3 circRNA isoforms across 12 human tissues. Details are as in (g).
