Fig. 4: TCDD and eGFP-SLC46A3 increases the copper chaperone for superoxide dismutase.

a Western blotting data show the level of copper-related proteins in the liver after oil (n = 5 per group) or TCDD (10 μg/kg for 7 days) treatment (n = 5 per group). Densitometrical analysis is shown in the right panel. b Western blotting data show the level of copper chaperone for superoxide dismutase (CCS) protein level after treatment with BCS (Cu (I) chelator in the media) for 3 days in the presence of eGFP-SLC46A3 in Hepa1c1c cells. Densitometrical analysis is shown in the right panel. NB nonboiled sample. c The level of heavy chain of ferritin, an indicator of labile cytoplasmic iron levels, was measured after induction of eGFP-SLC46A3 in Hepa1c1c7 cells. d SOD activity was measured when Hepa1c1c7 cells were treated with different doses of BCS in the presence of eGFP-SLC46A3. e Western blotting data show the level of copper chaperone for superoxide dismutase (CCS) in the livers from wild-type (WT)(n = 4 per group) and Slc46a3^−/− mice (n = 4 per group). Densitometrical analysis is shown in the right panel. f Expression of mRNAs encoding metal transporters in the mice liver after oil (n = 4 per group) or TCDD (10 µg/kg for 24 h) (n = 5 per group) administration. Each data point represents the mean ± SD and adjusted p value, presented in the panels, was determined by unpaired two-tailed Student’s t test using indicated sample sizes and groups.