Fig. 5: Slc46a3 affects mitochondrial potential and morphology.

a Fluorescence images of mitoTracker in the primary hepatocytes. Red color, mitochondria; blue color, nucleus; scale bar, 10 μm. The representative images were obtained from three independent experiments. b Relative mitochondrial sizes between WT and Slc46a3^−/−. c MitoTracker intensity in the primary hepatocytes in the presence or absence of mCherry-SLC46A3. The representative images were obtained from three independent experiments. N nucleus; scale bar, 10 μm. d MitoTracker intensity in the eGFP-SLC46A3-expressing Hepa1c1c7 cells. Scale bar, 10 μm; blue color, nucleus. The representative images were obtained from three independent experiments. e Mitochondrial potential images with TMRE dye in Hepa1c1c7 cells. The asterisk indicates the same location. The representative images were obtained from three independent experiments. f Mitochondrial oxygen consumption rate (OCR) in eGFP-SLC46A3-expressing Hepa1c1c1 cells. g Relative ATP levels were measured in the eGFP-SLC46A3-expressing Hep1c1c7 cells. h Transmission electron microscopy (TEM) images of hepatocytes in WT (n = 3 per group) and Slc46a3^−/− (n = 3 per group) mice. m mitochondria; scale bar, 500 nm. The representative images were obtained from three biologically independent liver samples. i TEM images of hepatocytes in eGFP-SLC46A3-expressing liver. The representative images were obtained from three biologically independent liver samples. ld lipid droplet, dm donut shape mitochondria, m mitochondria; scale bar, 500 nm. Each data point represents the mean ± SD and the adjusted p value, presented in the panels, was determined by unpaired two-tailed Student’s t test using indicated sample sizes and groups.