Fig. 6: TCDD induces accumulation of hepatic triglyceride through SLC46A3.

a Measurements of hepatic triglyceride (TG) and non-esterified fatty acid (NEFA) as well as serum ALT activity and serum ALP activity (n = 4 for WT-OIL group; n = 5 for other groups). Two-way ANOVA with Bonferroni’s multiple comparisons test, **p = 0.0062; NS not significant. b Representative Oil O Red staining of liver tissue. c Body weight changes after high-fat diet WT (n = 4 per group) and Slc46a3^−/− (n = 3 per group) mice. d Liver mass to body weight after high-fat diet in WT (n = 4 per group) and Slc46a3^−/− (n = 3 per group) mice. e Epididymal fat mass after high-fat diet in WT (n = 5 per group) and Slc46a3^−/− (n = 3 per group) mice. f Hepatic TG and NEFA levels after high-fat diet in WT (n = 3 per group) and Slc46a3^−/− (n = 5 per group) mice. g TG and NEFA levels in the serum after high-fat diet in WT (n = 4 per group) and Slc46a3^−/− (n = 4 per group) mice. NS not significant. h Hepatic mRNA expressions of lipid-related metabolizing genes by oil or TCDD (10 µg/kg for 24 h) in WT and Slc46a3^−/− mice. Two-way ANOVA with Bonferroni’s multiple comparisons test, ****p < 0.0001. The sample sizes of WT-OIL, WT-TCDD Slc46a3^−/−-OIL, and Slc46a3^−/−-TCDD are n = 4, 5, 5, 5 mice per group, respectively. Each data point represents the mean ± SD and the adjusted p value, presented in the panels, was determined by unpaired two-tailed Student’s t test using indicated sample sizes and groups.