Fig. 3: 1H-detected 2D and 3D correlation NMR spectra of F4-TPP+ bound S64V-EmrE in DMPC bilayers. | Nature Communications

Fig. 3: 1H-detected 2D and 3D correlation NMR spectra of F4-TPP+ bound S64V-EmrE in DMPC bilayers.

From: Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers

Fig. 3

All spectra were measured under 55 kHz MAS on CDN-labeled protein at a sample temperature of 285 K. a 2D 1H-15N correlation spectrum, showing assignment of selected resonances based on the 3D spectra. Blue assignments correspond to residues shown in b. b Representative 3D strips extracted from the four 1H-detected spectra, showing the assignment of residues T56–W63 of subunit A. Aliphatic 13C chemical shifts were assigned using the hCANH (orange) and hCA(CO)NH (green) experiments, whereas CO chemical shifts were assigned using the hCO(CA)NH (red) and hCONH (blue) experiments.

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