Fig. 5: Functional domain analysis of NOT4A.

a Schematic diagram showing position of point mutations in RING and RRM domains. Mutations in black are those introduced to the NOT4A gene, which correspond to equivalent conserved residues in ScNOT4 (shown in grey), additionally highlighted in Supplementary Fig. 1 sequence alignments. b anti-GUS western blot of steady state levels of pNOT4A::NOT4A-GUS variants expressed in not4a. Blots were repeated at least three times with similar results. c 10-day-old not4a and pNOT4A::NOT4A-GUS complementation lines grown on vertical plates ±10 µM lincomycin. Bar = 1 cm. d Representative rosette images of 3-week-old not4a and pNOT4A::NOT4A-GUS complementation lines under long day (LD) conditions. Bar = 1 cm.