Fig. 7: PGR3 can rescue the chloroplast-associated defects in not4a. | Nature Communications

Fig. 7: PGR3 can rescue the chloroplast-associated defects in not4a.

From: RETRACTED ARTICLE:The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation

Fig. 7

a Anti-YFP western blot of steady state PGR3-YFP protein levels in three independent Col-0 and not4a lines expressing pPGR3::PGR3-YFP. b Quantitative PCR (qPCR) of PGR3 in Col-0, not4a and the PGR3-YFP lines from a. Expression levels normalised to ACTIN7 and shown relative to Col-0 WT. n = 3 biological replicates. Error bars = SEM. c Confocal images of hypocotyl cells in Col-0 and not4a lines expressing pPGR3::PGR3-YFP. Panels show PGR3-YFP in green (left), chloroplasts in red (middle) and bright field view (right). Experiment was replicated at least 3 times (see Supplementary Fig. 7D for further repeats). d 10-day-old Col-0, not4a and PGR3-YFP transgenic seedlings grown on vertical plates ±10 µM lincomycin. Bar = 1 cm. e Representative rosette images of 4-week-old Col-0, not4a and not4a PGR3-YFP lines grown under long day (LD) conditions. Bar = 1 cm. f Cycloheximide (CHX) chase of PGR3-YFP and ACTIN in Col-0 and not4a ± bortezomib (BZ). g Yeast two-hybrid assay between NOT4A and PGR3. EV empty vector. P53/SV40 positive control. Growth on -LW confirms successful transformation; growth on -AHLW denotes interaction. Western blots in 7a and 7f were repeated three times with similar results.

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