Fig. 7: AR-42 restores Ghrh expression in Mll4-cKO mice.

a Schematics for AR-42 experiments. From 9 days after a plug was observed, vehicle or AR-42 was injected to pregnant dams daily, followed by embryo harvests at E12.5 or E17.5. b IHC analyses of relative levels of H3K4me1 and H3K27ac in embryos harvested at E12.5 as well as ISH analyses of Ghrh expression in embryos harvested at E17.5. Quantification of H3K4me1/H3K27ac levels in the developing ARC (arrows) done relative to the signals in the adjacent tissues (the trigeminal ganglion, indicated by asterisks). The number of mice used is as indicated below each genotype in parenthesis. Scale bars, 100 µm. c Embryos harvested at E15 (n = 3, each genotype and condition) were subject to ChIP analyses with IgG (controls) or antibodies against H3K4me1 or H3K27ac, followed by qPCR quantification of H3K4me1/H3K27ac levels on genomic region containing the Mll4/Nrf1 ChIP-seq peaks associated with Nrf1, Vat1, Flywch2, and Untr6 (negative control locus). Statistical differences were determined by two-sided Student’s t-test (b, c); *p < 0.05, **p < 0.01, ****p < 0.0001, and not significant (ns). Column bars represent mean, error bars indicate the SD (b, c).