Fig. 7: CRY1 regulates HR gene expression via binding to promoters and CRY1 correlates with HR gene expression in human disease.

a CRY1 was knocked down in C4-2-shCRY1 cells for 72 h. b ChIP-qPCR was performed to examine CRY1 binding after 30 mins and 4 h of 5 Gy IR treatment in C4-2 cells. Binding is plotted as percent of input. c CRY1 was knocked down in C4-2-shCRY1 cells for 72 h and cells were treated with 5 Gy IR for 4 h. C4-2 cells were treated with 10 µM KL001 for 6 h. d Co-expression of CRY1 and either ATM, MRE11A, and RAD50 mRNA in PCa tissue from publicly available data from MSKCC (Cancer Cell 2010). e Model summarizing the findings from this study. N = 3 independent experiments. Data are presented as mean values ± SEM and analyzed using Student’s t-test or two-way Anova (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Statistical significance was evaluated at 0.05 alpha level with GraphPadPrism, version 8.3.1, Mac. Source data are provided in the Source Data file.