Table 1 Statistics of cryo-EM data collection, refinement, and validation statistics.
From: Structural insights into TSC complex assembly and GAP activity on Rheb
TSC | |
|---|---|
Data collection and processing | |
Magnification | ×105,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 50 |
Defocus range (μm) | 1.0–3.5 |
Pixel size (Å) | 1.356 |
Symmetry imposed | C1 |
Initial particle images (No.) | 1,528,982 |
Final particle images (No.) | 131,022 |
Map resolution (Å) | |
Consensus reconstruction | 4.4 |
Focus wing-a reconstruction | 4.1 |
Focus core reconstruction | 3.6 |
Focus wing-b reconstruction | 3.9 |
FSC threshold | 0.143 |
Map resolution range (Å) | |
Consensus reconstruction | 4.0–20.0 |
Focus wing-a reconstruction | 4.0–20.0 |
Focus core reconstruction | 3.0–10.0 |
Focus wing-b reconstruction | 3.0–10.0 |
Refinement | |
Initial model used (PDB code) | 5EJC |
Model resolution (Å) | 4.5 |
FSC threshold | 0.5 |
Model resolution range (Å) | 4.0–4.5 |
Map sharpening B factor (Å2) | −129.92 |
Model composition | |
Non-hydrogen atoms | 23,934 |
Protein residues | 3089 |
Ligands | 0 |
B factors (Å2) | |
Protein | 141.96 |
Ligand | – |
RMS deviations | |
Bond lengths (Å) | 0.004 |
Bond angles (°) | 0.673 |
Validation | |
MolProbity score | 2.42 |
Clashscore | 24.52 |
Poor rotamers (%) | 0.12 |
Ramachandran plot | |
Favored (%) | 90.30 |
Allowed (%) | 9.70 |
Disallowed (%) | 0.00 |
