Fig. 5: circNDUFB2 provokes cellular immune responses. | Nature Communications

Fig. 5: circNDUFB2 provokes cellular immune responses.

From: circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity

Fig. 5

a Nine hundred and thirty-four dysregulated target genes in A549 cells with circNDUFB2 overexpression (circNDUFB2) versus control (Vector) were identified by RNA-seq analysis. Fold change > 2.0 or fold change < −2.0, P value < 0.05 as significant change. b Enrichment analysis for representative GO_BP and KEGG pathways in circNDUFB2 target genes. c GSEA analysis for the circNDUFB2 overexpression group (circNDUFB2) compared to the control group (Vector). NES normalized enrichment score, FDR false discovery rate. P values are calculated by permutation test. d qRT-PCR measured the fold change of indicated mRNAs in A549 and H1299 cells with or without circNDUFB2 overexpression. n = 3, 4 biologically independent samples, respectively. e Expression levels of indicated mRNA in H1650 cells with circNDUFB2 knockdown. n = 4 biologically independent samples. f, g ELISA measured the levels of CXCL10, CXCL11, CCL5, and IFNβ in the supernatants of A549 cells with circNDUFB2 overexpression (f) or H1650 cells with circNDUFB2 knockdown (g). n = 3, 4 biologically independent samples, respectively. Data are presented as mean ± s.d. in d–g. P values are calculated by unpaired two-sided t-test in a, and e–g.

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