Fig. 3: HilD derepresses mcpC by displacement of H-NS. | Nature Communications

Fig. 3: HilD derepresses mcpC by displacement of H-NS.

From: Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC

Fig. 3

a Competitive EMSA of the indicated promoter regions with purified MBP-HilD and H-NS. H-NS was added first (lanes 3–7) and increasing amounts of MBP-HilD was subsequently added (lanes 4–7). Similar results were obtained with n = 3 independent experiments. b–d GFP fluorescence over time. b WT bacteria containing the indicated reporter. Shown are the mean ± SD of n = 3 independent experiments. c Indicated strains harboring PmcpC (σ70) gfp. Shown are the mean ± SD of n = 3 independent experiments. d Indicated strains harboring PmcpC (σ70) gfp. H-NS up contains both PmcpC (σ70) gfp and pMPMT6 hnsWT. H-NS down contains both PmcpC (σ70) gfp and pMPMT6 hnsQ92am. Source data are provided as a Source Data file.

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