Table 1 RNA sequencing results comparing WT and hilDΔ3′UTR.

From: Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC

SL1344 Locus ID

Gene

Pathway

Log2 foldchange

p-value

padj

SL1344_RS05360

sopB

T3SS1

1.26

1E−13

3E−12

SL1344_RS14880

prgH

T3SS1

0.83

2E−05

2E−04

SL1344_RS14890

hilA

T3SS1

0.59

6E−04

0.003

SL1344_RS15005

invF

T3SS1

0.53

0.006

0.020

SL1344_RS06600

lpxR

T3SS1

1.22

4E−08

6E−07

SL1344_RS06605

hypoth.

Lipid A deacylase

1.45

0.008

0.024

SL1344_RS06610

hypoth.

Unknown

1.33

9E−09

1E−07

SL1344_RS22130

hypoth.

Endonuclease

1.39

4E−07

5E−06

SL1344_RS22135

hypoth.

Unknown

0.82

0.018

0.048

SL1344_RS22140

hypoth.

Bacteriophage prot

0.69

0.016

0.043

SL1344_RS16625

mcpC

Unknown

0.65

0.003

0.011

SL1344_RS09580

cheZ

Motility

0.11

0.325

0.466

SL1344_RS09585

cheY

Motility

0.13

0.327

0.467

SL1344_RS09590

cheB

Motility

0.15

0.157

0.270

SL1344_RS09615

motB

Motility

0.03

0.810

0.881

SL1344_RS09625

flhC

Motility

0.29

0.149

0.259

SL1344_RS09630

flhD

Motility

0.26

0.210

0.337

SL1344_RS09855

fliG

Motility

0.03

0.902

0.945

SL1344_RS09900

fliP

Motility

0.11

0.633

0.757

SL1344_RS09910

fliR

Motility

0.20

0.527

0.667

SL1344_RS16230

mcpA

Motility

0.19

0.343

0.486

  1. Shown are selected genes of the T3SS1, previously identified HilD targets and all upregulated motility genes. n = 3 independent samples were collected from each strain. A positive log fold change indicates expression is up in hilDΔ3′UTR compared to WT. Significant gene expression differences were identified using DESeq2; p values shown were calculated using the Wald test and the p value was adjusted (padj) for multiple testing using the Benjamini and Hochberg procedure. Full RNAseq results are found in Supplementary Data 1.
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