Fig. 3: Localization of actomyosin cables in three-dimension.
From: Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion
a Immunostaining of MDCK cells extruding from monolayer (extruding cell at the center), DAPI channel, Merged of DAPI and AlexaFluor-568 channel, Myosin staining color-coded according to the height of the monolayer. The contrasting localization of myosin around nucleus at two separated planes (~3 µm versus 7 µm at each side) suggested the partitioning of the purse-string into two rings which are tilted. Differential localization of actomyosin cables were observed in 34 extrusion events from 4 independent experiments. b 3D reconstruction for images in a. A circular fit was performed on each ring on 3D reconstruction images. The angle at which the circle formed with horizontal plane xy was defined as shown on the image. c Histogram of distribution of angles that the purse-string ring forms w.r.t horizontal plane. (WT: n = 32 rings in m = 19 extrusion events; NSC-23766 (Rac-inhibitor): n = 10 rings in m = 11 extrusion events). Source data are provided as a Source Data file.
