Fig. 5: Hyperactivation of AURKB mediates DEN-induced abscission failure.

a Schema for detection of AURKB and pT232-AURKB in hepatocyte primary culture: Injection of normal saline or DEN at p15 followed by mice sacrifice at p25 for primary culture. Cultured hepatocytes were treated with AZD1152 or equal amount DMSO. b Immunofluorescence of pT232-AURKB and AURKB during abscission in indicated cultured hepatocytes with or without 50 nM AZD1152 treatment. The morphology of nuclei and intercellular bridges were outline by counterstaining with tubulin and DAPI, respectively. High magnification shows the detailed structure of the midbodies. c, d Quantification of the intensity of pT232-AURKB, AURKB, and AURKB normalized pT232-AURKB at the midbody from (b). The length of intercellular bridge was quantified by analyzed the highly condensed tubulin signal between daughter cells. n = 115, 100, and 100 dividing hepatocytes at abscission stage, respectively, per group from three independent experiments. e Quantification of the abscission failure ratio in the indicated cultured hepatocytes from time-lapse images. n > 500 dividing hepatocytes/group, four independent experiments/group. Statistic: one-way ANOVA (c–e). Values represent the mean ± SEM (also see Supplementary Fig. 5).