Fig. 6: Hyperactivation of AURKB drives DEN-induced hyperpolyploidization of hepatocytes.
From: Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liver
a Pharmacological manipulations of AURKB activity, according to the schema in Supplementary Fig. 6a, changes the nucleus size of cultured hepatocytes. Cultured hepatocytes were treated with 50 nM AZD1152 or equal amount DMSO. Nucleus and cell morphology were outlined by DAPI (cyan) and tubulin (red), respectively. b, c Frequency distribution and dot plot graphs display the nuclear sizes of indicated cultured hepatocytes at different time points. Line graph shows the nucleus size in indicated cultured hepatocyte between 48 and 96 h. n = 500 hepatocytes/group from four independent experiments. d Representative images show nuclear budding (white arrow) and micronuclei (yellow arrow) in indicated cultured hepatocytes. e, f The percentage of micronuclei and nuclear budding positive cell and the number of micronuclei in a cell of indicated cultured hepatocytes. n = 100/150/150 (Ctrl), 125/150/150 (DEN), and 150/200/200 (DEN + AZD) cells, respectively. Statistic: one-way ANOVA (b); two-way ANOVA (c), (e), and (f). Values represent the mean ± SEM (also see Supplementary Fig. 6).
