Fig. 4: High variation in SH-P regions in diverse S. pombe strains.

a Phylogenetic relationships among 972 and JB strains based on genome-wide sequences according to a previous study by Tusso et al.³⁰. Locations where the strains were collected are indicated³³. ND, not defined. Regarding 972, locations where the strains are stocked and analyzed are indicated in parentheses. b Schematics of the SH-P sequences in strain JB22 (972) with common segments. Sequences of indicated segments exhibit 90% or higher identities with those of PomBase or 972SD4. Black bars indicate relatively long regions that show the same segmental arrangements with those of 972SD4 and PomBase-SH2R. c Schematics of the SH-P sequences in strain JB760. Second bar shows an SH-P region of SH3L and/or SH3R of Ch3. Contigs 1–3 include SH-P regions that are not allocated to specific subtelomeres. d Schematics of the SH-P sequences in strain JB1174. C′′ is a subtype of segment C which contains six units of c motifs (see Figs. 3b and 4i). ∆ indicates a partial sequence of each segment with no less than 10 bp decrease in total length. “?” indicates 75–90% sequence identity with the corresponding segment. e Schematics of the SH-P sequences in strain JB934. C′′′ and C′′′′, subtypes of segment C, contain seven and eight units of c motifs, respectively (see Figs. 3b and 4i). SH1R′ and SH2R′ (highlighted in red) indicate that they are located at subtel2R and subtel1R in JB934, but overall sequences of telomere-side regions of Ch2R and Ch1R are homologous to those Ch1R and Ch2R in 972, respectively (see Supplementary Fig. 4). Dark gray box in SH1R′ indicates a sequence that has not been found in 972 strains and is not shared by the subtelomeres in other JB strains. f Schematics of the SH-P sequences in strain JB858. Contig 1 includes an SH-P region that is not allocated to a specific subtelomere. g Schematics of the SH-P sequences in strain JB873. Black boxes indicate common segments shared by some of JB strains, although their sequences have not been found in 972 strains (see Supplementary Fig. 5 for their sequences). Note that a partial LTR sequence is inserted at SH2L. h Schematics of the SH-P sequences in strain JB1197. i Subtypes of segment C. Note that the types of c motifs are not specified due to the relatively low reliability of sequences by long-read DNA sequencing.