Fig. 4: IL-12:Fc reshapes the endogenous compartment within the glioma TME.

A Umap showing the FlowSOM-guided metaclustering gated on TCR-β⁺ T cells from live CD45⁺ cells and heatmap showing the median marker expression for each defined metacluster (value range: 0–1) and Pie charts represent relative frequencies for the three TCR-β⁺ T cell subclusters among total TCR-β⁺ T cells within the different conditions (NT+PBS n = 4, NT+IL-12:Fc n = −5, CAR+PBS n = 5, CAR+IL-12:Fc n = 5 mice, representative of n = 2 independent experiments). B Median expression of selected cell markers shown for all TCR-β⁺ T cell subclusters: CD4, CD8, and T_regs, (NT+PBS n = 4, NT+IL-12:Fc n = 5, CAR+PBS n = 5, CAR+IL-12:Fc n = 5 mice, representative of n = 2 independent experiments). C t-SNE map showing the FlowSOM-guided metaclustering on CD11b⁺CD45⁺ cells and heatmap showing the median marker expression for each defined metacluster (value range: 0–1) and Pie charts represent relative frequencies for the nine CD11b⁺CD45⁺ cell subclusters among total CD11b⁺CD45⁺ cells within the different conditions, (NT+PBS n = 4, NT+IL-12:Fc n = 5, CAR+PBS n = 5, CAR+IL-12:Fc n = 5 mice, representative of n = 2 independent experiments). Data are presented as mean values ± SEM. Ordinary One-way Anova with Dunnett’s multiple comparison test (B). Source data are provided as a Source Data file.