Fig. 3: Distinct molecular mechanisms regulating γ2-GIM’s specific interactions with GABARAP or AP2.

a The ribbon representation model showing the overall structure of the previously reported AP2–γ2-GIM complex. The figure is generated using the previous reported peptide complex structure (PDB code 2PR9²⁸). In this drawing, AP2 is shown in cyan and γ2-GIM is shown in orange. b ITC-derived binding curves comparing the binding affinities between AP2 and GABA_AR γ2-GIM or its five mutant variants (E402T, Y403E, Y405E, E406R, and L408Q). c Compare the conformations of γ2-GIM in complex with GABARAP or AP2 by superimposing the “YECL” motif. d Bar graph of fold changes of K_d values compared to WT showing the impacts on binding resulting from mutations of key residues in the GABA_AR γ2-GIM. Titrations for WT and E406R mutant were performed three times; others were performed once. K_d values are reported in Supplementary Table 2.