Fig. 5: GABARAP functions to stabilize GABAARs via promoting its trafficking pathway. | Nature Communications

Fig. 5: GABARAP functions to stabilize GABAARs via promoting its trafficking pathway.

From: Structural basis of GABARAP-mediated GABAA receptor trafficking and functions on GABAergic synaptic transmission

Fig. 5

a Representative trace records and average values of GABA currents activated by 1 mM GABA in HEK-293 cells co-expressing GABAAR (α1β2γ2) and WT or mutant GABARAP, with or without treatment using the endocytosis inhibitor dynasore (80 μM, 2 h). n = 10, 9, 12, 8, and 9 of each group (from left to right). Data are represented as the mean ± SEM. **P = 0.0060, GABAAR vs. GABAAR+Dynasore; **P = 0.0070, GABAAR+Dynasore vs. GABAAR+GABARAP+Dynasore based on one-way ANOVA; ns, not significant (P > 0.05). b Representative trace records and average values of GABA currents activated by 1 mM GABA in HEK-293 cells co-expressing GABAAR (α1β2γ2) and GABARAP with or without treatment of the protein trafficking inhibitor Brefeldin A (5 μg/mL, 30 min). n = 14, 10, 9, and 10 of each group (from left to right). Data are represented as the mean ± SEM. *P = 0.0431, GABAAR vs. GABAAR+Brefeldin A; ***P = 0.0001, GABAAR vs. GABAAR+GABARAP; ***P = 0.0001, GABAAR+GABARAP vs. GABAAR+GABARAP+Brefeldin A based on one-way ANOVA; ns not significant (P > 0.05).

Back to article page