Fig. 6: Differential phosphorylation modulates the capacity of GABAA receptor γ2 subunit to interact with GABARAP vs. the clathrin adaptor protein AP2. | Nature Communications

Fig. 6: Differential phosphorylation modulates the capacity of GABAA receptor γ2 subunit to interact with GABARAP vs. the clathrin adaptor protein AP2.

From: Structural basis of GABARAP-mediated GABAA receptor trafficking and functions on GABAergic synaptic transmission

Fig. 6

a ITC-derived binding curves comparing binding affinities between AP2 and a synthesized γ2-GIM WT peptide or 3 phopho-peptides (pY403, pY405, and pY403pY405). b ITC-derived binding curves comparing binding affinities between GABARAP and the synthesized γ2-GIM WT peptide or 3 phopho-peptides (pY403, pY405, and pY403pY405). c Effects of Src kinase on GABAAR-mediated current densities in HEK-293 cells co-expressing GABARAP. Representative trace records and average values of GABA currents activated by 1 mM GABA in HEK-293 cells co-expressing GABAAR (α1β2γ2), GABARAP, and Src kinase with or without treatment of kinase inhibitor PP2. n = 10, 9, 10, and 10 of each group (from left to right). Data are represented as the mean ± SEM. ***P = 0.0001, GABAAR vs. GABAAR+GABARAP; ***P = 0.0001, GABAAR vs. GABAAR+GABARAP+Src kinase+PP2 based on one-way ANOVA; ns not significant (P > 0.05).

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