Fig. 4: CREPT maintains the proliferation and differentiation of Lgr5⁺ ISCs.

a Gate strategy of sorting Lgr5-GFP^high cells from TAM treated WT and Lgr5-CREPT^KO mice. b Volcano plot showing the upregulated and downregulated genes in Lgr5-CREPT^KO cells compared to Lgr5-CREPT^WT ones. c, d GO analysis of downregulated genes (c) and upregulated genes (d) in CREPT deleted Lgr5⁺ stem cells, respectively. e Relative FPKM showing the expression of differentiation-related genes in WT and Lgr5-CREPT^KO cells. Data represent mean of 2 mice per genotype derived from RNA-seq analysis. f Heatmap showing the expression of EMT-related genes of WT and Lgr5-CREPT^KO cells. n = 2 mice for each genotype. g Heatmap showing the relative expression level of cell cycle promoting genes of WT and Lgr5-CREPT^KO cells. n = 2 mice for each genotype. h Representative images of EdU-labeled cells in 4OHT treated intestinal organoids derived from WT and Lgr5-CREPT^KO mice. Images are representative of at least three independent experiments. Scale bars: 30 μm.