Fig. 5: CREPT promotes intestinal regeneration through the Wnt signaling pathway.
From: CREPT is required for murine stem cell maintenance during intestinal regeneration
a Volcano plot showing the upregulated and downregulated genes in irradiated (IR)-Vil-CREPTKO crypts compared to IR-Vil-CREPTWT ones. b Quantitative RT-PCR analysis of Wnt target genes in irradiated WT and Vil-CREPTKO intestines at 3 dpi. n = 3 independent experiments. c Heatmap showing the expression levels of Wnt target genes in untreated/irradiated WT and Vil-CREPTKO intestines. d Quantitative RT-PCR analysis of Cd44, Ascl2, EphB3, and Sox9 expression in untreated/irradiated WT and Vil-CREPTKO intestines. p < 0.0001 by 2-tailed Student’s t-test. n = 3 independent experiments. e ChIP-seq signals of CREPT occupied genomic loci. The CREPT occupancy frequency is illustrated at the genome of Wnt target genes including Cd44, EphB1, Alcam, and Rspo2. f Quantitative PCRs showing the enrichment of CREPT on the promotor and termination region of the Cd44 gene. The primers A, B, and C, were designed as indicated. Images are representative of three independent experiments. Statistics data represent mean ± SEM.
