Fig. 3: Demonstration of typhoid- and paratyphoid-specific toehold switches.
From: A glucose meter interface for point-of-care gene circuit-based diagnostics
a Glucose output from the detection of RNA sequences related to typhoid (Typh), paratyphoid A (Para A), paratyphoid B (Para B), and fluoroquinolone resistance (Q). CFS background (N, no switch/trigger), switch alone (S, no trigger), and switch with target (S + T) are shown for each experiment. Typh, Para A, Para B switches at 312.5 pg/µL and targets at 50 nM. Q switch at 1.25 ng/µL and target at 100 nM. For each set 3 technical replicates are shown, representative of 3 independent experiments. b NASBA amplification of STY RNA at the given initial concentrations followed by glucose generation reaction. Typh switch at 1.25 ng/µL. *p = 0.0460; ****p < 0.0001. 3 technical replicates are shown, representative of 3 independent experiments. c RNA target(s) corresponding to typhoid (“STY”) and fluoroquinolone resistance (“Q”) were first amplified in a combined NASBA reaction, followed by multiplexed glucose reactions containing trehalase switches for both targets. Typh switch at 1.25 ng/µL, Q at 2.5 ng/µL. *: STY Trig vs. None p = 0.0180, STY Trig + Q Trig vs. Q Trig p = 0.0128; ***p = 0.0003. d Web-based interface for automated interpretation of the multiplexed diagnostic data presented in c. The user inputs the glucose measurements for a negative control and their experimental sample. Following submission, the website compares the data with known results to provide an interpretation of results (here typhoid positive). ns: not significantly different. All data are presented as mean values ± SD. Source data are provided as a Source Data file.
