Fig. 2: Kinetics and specificity of SARS-CoV-2 S protein binding to hACE2 receptor determined by bio-layer interferometry (BLI) and ELISA. | Nature Communications

Fig. 2: Kinetics and specificity of SARS-CoV-2 S protein binding to hACE2 receptor determined by bio-layer interferometry (BLI) and ELISA.

From: SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in mice

Fig. 2

BLI sensorgram showing the binding of a wild-type (WT) SARS-CoV-2 S, b BV2365, and c NVX-CoV2373 spike proteins to histidine-tagged hACE2 receptor immobilized on a Ni-NTA biosensor tip. Data are shown as colored lines at different concentrations of spike protein. Red lines are the best fit of the data. d WT-SARS-CoV-2 S, e BV2365, and f NVX-CoV2373 demonstrated binding to hACE2 receptor but failed to bind hDPP4 as determined by ELISA. Source data are provided as a Source Data file.

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