Fig. 3: Stability of SARS-CoV-2 variants under stress conditions. | Nature Communications

Fig. 3: Stability of SARS-CoV-2 variants under stress conditions.

From: SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in mice

Fig. 3

The hACE2 receptor binding ELISA method was used to assess the structural integrity of BV2365 and NVXCoV2373 under stressed conditions. a NVXCoV2373 and b BV2365 were exposed to repeat freeze–thaw cycles, pH extremes, agitation, elevated temperatures, and oxidation for extended periods as indicated. Treated samples were immobilized on 96-well plates, then incubated with serially diluted (2–0.0001 μg mL−1) histidine-tagged hACE2. Bound receptor was detected with HRP-conjugated rabbit anti-histidine IgG. Source data are provided as a Source Data file.

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