Fig. 1: Keratinocyte differentiation involves IpA alterations and CPSF downregulation.

a Illustration showing the workflow of analyzing IpA sites in undifferentiated (UD) and differentiated (DF) keratinocytes. b–d Scatter plots showing the correlation of the 3′READS+ data between replicates, and between undifferentiated and differentiated conditions. e Heat map showing fold change of the 428 differentially used IpA sites during keratinocyte differentiation, in both 3′READS+ data and RNA-seq data. f, g Genome browser tracks showing the differential IpA usage in both EPSN and IQCK during keratinocyte differentiation, with both 3′READS+ and RNA-seq data. (Beige highlight: 3′UTR; Green highlight: UD-enriched IpA; Red highlight: DF-enriched IpA). h Illustration showing the composition and function of the CPSF complex. h Heat map comparing the relative mRNA levels of CPSF subunits and POLR2A between undifferentiated and differentiated keratinocytes. i qRT-PCR comparing the mRNA expression of CPSF genes between undifferentiated versus differentiated keratinocytes. Dots represent data points in technical replicates. j Immunoblots comparing the expression of CPSF subunits at the protein level between undifferentiated and differentiated human keratinocytes. β-tubulin was used as loading control. Average fold change and SD quantified from 3 replicates are indicated blow each panel. Source data are provided as a Source Data file.