Fig. 4: CPSF Downregulation Partially Accounts for the IpA Alterations in Differentiation. | Nature Communications

Fig. 4: CPSF Downregulation Partially Accounts for the IpA Alterations in Differentiation.

From: Epidermal progenitors suppress GRHL3-mediated differentiation through intronic polyadenylation promoted by CPSF-HNRNPA3 collaboration

Fig. 4

a Pie chart showing 178 out of the 428 IpA sites altered in differentiation are influenced by CPSF1 knockdown. b Heat map showing the fold changes of these 178 IpA sites, comparing DF versus CPSFi. c–f Genome browser tracks comparing the differential IpA usage in DF and in CPSFi. Locations of the qPCR primers designed for IpA usage quantification are labelled below (brown arrowheads: proximal primers, green arrowheads: distal primers, beige highlight: 3′UTR, green highlight: UD-enriched IpA). g–j qRT-PCR quantification of the relative usage of the IpAs is calculated using the Proximal:Distal usage ratio, comparing undifferentiated vs. differentiated keratinocytes, CSPF1 siRNA and CPSF CRISPRi vs. control conditions, and MYC knockdown vs. control. Dots represent data points in technical replicates. Source data are provided as a Source Data file.

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