Fig. 6: HNRNPA3 cooperates with CPSF to promote GRHL3 IpA. | Nature Communications

Fig. 6: HNRNPA3 cooperates with CPSF to promote GRHL3 IpA.

From: Epidermal progenitors suppress GRHL3-mediated differentiation through intronic polyadenylation promoted by CPSF-HNRNPA3 collaboration

Fig. 6

a Illustration showing the genetic screen for identifying factors that enhance GRHL3 IpA. b Heat map showing the knockdown efficiency of the two shRNAs targeting specific candidates that include potential CPSF1-interacting proteins as well as CSTF2. c Heat maps showing the relative fold change of GRHL3 IpA usage comparing double knockdown vs CPSF single knockdown. d qRT-PCR showing the fold change of GRHL3 IpA usage, comparing HNRNPA3-CPSF double knockdown, single knockdowns and control. Dots represent data points in technical replicates. e, f Co-immunoprecipitation showing CPSF1 associates with HNRNPA3 in undifferentiated condition, but not in differentiated condition. This association is not disrupted by RNase treatment. Quantification of immunoprecipitation efficiency is indicated below. Source data are provided as a Source Data file.

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