Fig. 1: Steroid 5α-reductase activity of PbSRD5A.
From: Crystal structure of steroid reductase SRD5A reveals conserved steroid reduction mechanism
a Steroid 5α-reductase family proteins can reduce Δ4 carbon–carbon double bound to form α configuration at C5 of products. These reactions can be inhibited by finasteride and dutasteride specifically. b Testosterone (T), 4-androstene-3,17-dione (AD), and progesterone (P) metabolism in vitro and in HEK 293 T cell. Purified PbSRD5A and 293 T cells transiently transfected with PbSRD5A were treated with [3H]-labeled T, AD, and P for 1 h and 12 h, respectively. n.s. means no signal. c Time curve of reduction reaction from progesterone to 5α-DHP by PbSRD5A. d Inhibition of PbSRD5A by finasteride (Fina) and dutasteride (Duta) in vitro, both with the concentration of 1 μM and 10 μM, respectively. e Dutasteride inhibits the activity of PbSRD5A in vitro, with an IC50 value of 1.59 ± 0.19 μM (logIC50 = 0.20 ± 0.05). b–e The percentages of products were measured using high-performance liquid chromatography (HPLC) with detected radioactivity. Data are mean±s.d. derived from technically independent experiments in duplicate. Each experiment was reproduced three times on separate occasions with similar results.
