Fig. 3: Loss of RUNX1 or SMAD3 induces cell identity switch.
From: Core transcription regulatory circuitry orchestrates corneal epithelial homeostasis
a Heatmaps of differentially expressed genes produced by RUNX1 or SMAD3 KD. Red mark represents SE-assigned genes. b GO BP analysis for the upregulated genes in RUNX1-depleted and SMAD3-depleted LSCs (pvalueCutoff = 0.01 and qvalueCutoff = 0.05). c GSEA for genes that (i) are expressed at higher levels in LSCs than in SESCs and (ii) are more highly expressed in SESCs than in LSCs. NES: normalized enrichment score. d Schema representation of the air-lifting culture system. LSCs were seeded in the transwell inserts and incubated in medium until they were confluent. Then, the medium in the upper chamber was removed to induce differentiation into a stratified epithelium sheet. e Hematoxylin and eosin (H&E) and immunofluorescence staining of the indicated genes in the differentiated corneal epithelium sheet after air-lifting induction. Scale bars, 50 μm. f Immunofluorescence staining of the indicated genes in the differentiated corneal epithelium sheets treated with the indicated shRNAs. Scale bar, 50 μm.
