Fig. 7: Dio2Ala92 mice are protected from osteoarthritis.
From: Accelerating functional gene discovery in osteoarthritis
a 16-week-old mice with Dio2 polymorphisms homologous to the Thr92Ala polymorphism in human DIO2 (Dio2Thr92, n = 9 and Dio2Ala92, n = 10) were analyzed by iodine contrast-enhanced μCT (ICEμCT), joint surface replication (JSR), and subchondral X-ray microradiography (scXRM). b ICEμCT images of medial tibial plateau articular cartilage (red) and subchondral bone (blue). Graphs show articular cartilage volume (Cg.V) and median articular cartilage thickness (Median Cg.Th). c Back-scattered electron-scanning electron microscopy images of medial tibial plateau JSRs. Graph shows articular cartilage (Cg) damage. d ScXRM images of proximal tibia and the medial tibial plateau subchondral bone region of interest (dashed box) in which greyscale images are pseudocoloured according to a 16 color look-up table in which low bone mineral content (BMC) is yellow and high BMC is pink. Graph shows subchondral BMC (SC BMC). Scale bars = 100 μm (b, c) and 1 mm (d). *P < 0.05, **P < 0.01, two-tailed Wilcoxon rank sum test. Cg.V: P = 0.019, Median Cg.Th: P = 0.006, Cg. Damage area: P = 0.016. Source data are provided as a Source Data file.
