Fig. 6: The activation-induced cytokine and chemokine genes show “open” chromatin configuration before MCs encounter antigen and show increased chromatin accessibility and H3K27ac modification in response to IgE receptor crosslinking.

a Representative tracks from resting MCs. b Enrichment distribution analysis of chromatin accessibility at the TSS of the cytokine and chemokine genes. c Representative tracks from resting (UN) and activated (ST) BMMCs. d Calculated average reads at enhancers per kb per enhancer in and activated BMMCs. e The regulatory potential scores were calculated using either H3K27ac ChIP-seq peaks or Omni-ATAC-seq peaks. Data a–e represent two biological samples. f Calculated average FPKM reads of RNA transcripts in and activated BMMCs (n = 3 biologically independent samples). The IGV tracks are generated from one biological sample, representing two biological replicates with similar patterns. Middle line inside each box represents the median, upper and lower bounds of the box represent the third and first quartiles, respectively. Whiskers represent 1.5 times of the interquartile range. P-values were calculated by a two-tailed student’s t test without adjustments.